In Vivo Imaging Systems
With over 20 years of experience Berthold Technologies is one of the pioneers of in vivo imaging. Our first system, the Luminograph enabled scientists to detect low light emission in organisms, opening up the opportunity of monitoring reporter genes in animals and plants in a non-invasive way. This new and exciting technology changed medical research by providing the option to work under physiological conditions in a living animal. Today, it is possible to perform non-invasive in vivo imaging of specific molecular and cellular mechanisms.
Multiple molecular events can be monitored simultaneously, for example to visualize drug effects, to optimize drug and gene therapy, to monitor tumor growth or to study disease progression in both, living animals and plants. By providing insights into transcription–translation feedback loops, which are entrained by environmental signals such as light or temperature, even circadian clocks in plants can be monitored.
Learn more on how to gain real insights with our in vivo imaging systems by translating your in vitro data into clinical applications, and by marrying cell biology with physiology.
The NightOWL II In Vivo Imaging System is the first imager with a motor-driven camera inside the cabinet. Optimum resolution and focus of the sample are achieved by automatic positioning of the camera according to the actual sample size. The camera can be moved from a height of 50 mm to 725 mm allowing focussing on every sample size up to 250 mm. For close-ups a macro table can be used. The camera is set up with flat field and height correction. This calibration eliminates non-uniformities caused by variations in the optical path due to height, illumination or lens effects.
To cover the broadest range of in vivo imaging applications, the NightOWL II provides a wide variety of accessories, including heating tables to maintain optimum body temperature, gas anaesthesia unit, animal isolation chambers for 1 or 5 rodents, animal beds for multimodality imaging, and more.
On the other hand, the NightShade has been developed for in vivo plant imaging: it includes LED lighting that, combined with the powerful scheduler functions of its software, allows to create a realistic daylight simulation. To monitor gene expression in roots and shoots and correlate to growth morphology, the laterally mounted camera and the rotating table holding the trays with the seedlings in a vertical orientation can be used not to disturb the geotropic behaviour of the seedlings. A flange with light-tight ports allows for the introduction of light guides, cables or tubings, e.g. to water the plants inside during long imaging experiments, that may extend for days or even weeks.